Roukos Lab
Research Publications Group Members Biography2024
Longo GMC*, Sayols S*, Kotini AG, Heinen S, Möckel MM, Beli P and Roukos V ( 2024 ) Linking CRISPR-Cas9 double-strand break profiles to gene editing precision with BreakTag. Nat Biotechnol , doi: 10.1038/s41587-024-02238-8 (*indicates joint contribution) Link
Longo GMC*, Sayols S*, Stefanova ME*, Xie T*, Elsayed W*, Panagi A, Stavridou AI, Petrosino G, Ing-Simmons E, Melo US, Gothe HJ, Vaquerizas JM, Kotini AG, Papantonis A#, Mundlos S# and Roukos V# ( 2024 ) Type II topoisomerases shape multi-scale 3D chromatin folding in regions of positive supercoils. Mol Cell , doi: 10.1016/j.molcel.2024.10.007 (*indicates joint contribution, #indicates joint correspondence) Link
Zhang N*, Harbers L*, Simonetti M*, Diekmann C, Verron Q, Berrino E, Bellomo SE, Longo GMC, Ratz M, Schultz N, Tarish F, Su P, Han B, Wang W, Onorato S, Grassini D, Ballarino R, Giordano S, Yang Q, Sapino A, Frisén J, Alkass K, Druid H, Roukos V, Helleday T, Marchiò C, Bienko M and Crosetto N ( 2024 ) High clonal diversity and spatial genetic admixture in early prostate cancer and surrounding normal tissue. Nat Commun , 15:3475 (*indicates joint contribution) Link
2023
Petropoulos M, Champeris Tsaniras S, Nikou S, Maxouri S*, Dionellis VS*, Kalogeropoulou A, Karamichali A, Ioannidis K, Danalatos IR, Obst M, Naumann R, Delinasios GJ, Gorgoulis VG, Roukos V, Anastassiadis K, Halazonetis TD, Bravou V, Lygerou Z# and Taraviras S# ( 2023 ) Cdt1 overexpression drives colorectal carcinogenesis through origin overlicensing and DNA damage. J Pathol , 259:10–20 (*indicates joint contribution, #indicates joint correspondence) Link
2022
Abu-Libdeh B*, Jhujh SS*, Dhar S, Sommers JA*, Datta A, Longo GM, Grange LJ, Reynolds JJ, Cooke SL, McNee GS, Hollingworth R, Woodward BL, Ganesh AN, Smerdon SJ, Nicolae CM, Durlacher-Betzer K, Molho-Pessach V, Abu-Libdeh A, Meiner V, Moldovan GL, Roukos V, Harel T, Brosh Jr RM and Stewart GS ( 2022 ) RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase RECQL1. J Clin Invest , 132:e147301 (*indicates joint contribution) Link
Mosler T*, Baymaz HI*, Gräf JF, Mikicic I, Blattner G, Bartlett E, Ostermaier M, Piccinno R, Yang J, Voigt A, Gatti M, Pellegrino S, Altmeyer M, Luck K, Ahel I, Roukos V and Beli P ( 2022 ) PARP1 proximity proteomics reveals interaction partners at stressed replication forks. Nucleic Acids Res , 50:11600–11618 (*indicates joint contribution) Link
Tsaridou S, Velimezi G, Willenbrock F, Chatzifrangkeskou M, Elsayed W, Panagopoulos A, Karamitros D, Gorgoulis V, Lygerou Z, Roukos V, O’Neill E# and Pefani DE# ( 2022 ) 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling. EMBO Rep , 23:e544833 (#indicates joint correspondence) Link
2021
Longo GMC and Roukos V ( 2021 ) Territories or spaghetti? Chromosome organization exposed. Nat Rev Mol Cell Biol , 22:508–508 Link
Mosler T, Conte F, Longo GMC, Mikicic I, Kreim N, Möckel MM, Petrosino G, Flach J, Barau J, Luke B, Roukos V and Beli P ( 2021 ) R-loop proximity proteomics identifies a role of DDX41 in transcription-associated genomic instability. Nat Commun , 12:7314 Link
Papantonis A and Roukos V ( 2021 ) Cohesin puts a break on distal homology hunt. Nat Cell Biol , 23:1112–1114 Link
Zhang S*, Übelmesser N*, Josipovic N*, Forte G, Slotman JA, Chiang M, Gothe HJ, Gusmao EG, Becker C, Altmüller J, Houtsmuller AB, Roukos V, Wendt KS, Marenduzzo D and Papantonis A ( 2021 ) RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis. Sci Adv , 7:eabg8205 (*indicates joint contribution) Link
2020
Bouwman BAM, Agostini F, Garnerone S, Petrosino G, Gothe HJ, Sayols S, Moor AE, Itzkovitz S, Bienko M, Roukos V and Crosetto N (2020) Genome-wide detection of DNA double-strand breaks by in-suspension BLISS. Nat Protoc, 15:3894–3941 Link
2019
Gothe HJ, Bouwman BAM, Gusmao EG, Piccinno R, Petrosino G, Sayols S, Drechsel O, Minneker V, Josipovic N, Mizi A, Nielsen CF, Wagner EM, Takeda S, Sasanuma H, Hudson DF, Kindler T, Baranello L, Papantonis A, Crosetto N and Roukos V (2019) Spatial chromosome folding and active transcription drive DNA fragility and formation of oncogenic MLL translocations. Mol Cell, 75:267-283.e12 Link
Lucic B*, Chen HC*, Kuzman M*, Zorita *E, Wegner J, Minneker V, Wang W, Fronza R, Laufs S, Schmidt M, Stadhouders R, Roukos V, Vlahovicek K#, Filion GJ# and Lusic M# (2019) Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration. Nat Commun, 10:4059 (*indicates joint contribution, #indicates joint correspondence) Link
Piccinno R, Minneker V and Roukos V (2019) 53BP1—DNA repair enters a new liquid phase. EMBO J, 38:e102871 Link
2018
Dörsam B, Seiwert N, Foersch S, Stroh S, Nagel G, Begaliew D, Diehl E, Kraus A, McKeague M, Minneker V, Roukos V, Reißig S, Waisman A, Moehler M, Stier A, Mangerich A, Dantzer F, Kaina B and Fahrer J (2018) PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression. Proc Natl Acad Sci USA, 115:E4061–E4070 Link
Gothe HJ, Minneker V and Roukos V (2018) Dynamics of double-strand breaks: Implications for the formation of chromosome translocations. Pages 27–38 in: Chromosome Translocation; Advances in Experimental Medicine and Biology, vol. 1044 (ed. Zhang Y), Springer, Singapore Link
Roukos V (2018) Actin proteins assemble to protect the genome. Nature, 559:35–37 Link
Zirkel A, Nikolic M, Sofiadis K, Mallm JP, Brackley CA, Gothe H, Drechsel O, Becker C, Altmüller J, Josipovic N, Georgomanolis T, Brant L, Franzen J, Koker M, Gusmao EG, Costa IG, Ullrich RT, Wagner W, Roukos V, Nürnberg P, Marenduzzo D, Rippe K and Papantonis A (2018) HMGB2 loss upon senescence entry disrupts genomic organization and induces CTCF clustering across cell types. Mol Cell, 70:730–744.e6 Link
2017
Piccinno R, Cipinska M and Roukos V (2017) Studies of the DNA damage response by using the Lac operator/repressor (LacO/LacR) tethering system. Pages 263–275 in: ATM Kinase; Methods in Molecular Biology, vol. 1599 (ed. Kozlov S V), Humana Press, New York Link
2015
Roukos V and Mathas S (2015) The origins of ALK translocations. Front Biosci, 7:260–268 Link
Roukos V#, Pegoraro G, Voss TC and Misteli T# (2015) Cell cycle staging of individual cells by fluorescence microscopy. Nat Protoc, 10:334–348 (#indicates joint correspondance) Link
2014
Dittmer TA, Sahni N, Kubben N, Hill DE, Vidal M, Burgess RC, Roukos V and Misteli T (2014) Systematic identification of pathological Lamin A interactors. Mol Biol Cell, 25:1493–1510 Link
Roukos V#, Burgess RC and Misteli T# (2014) Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc, 9:2476–2492 (#indicates joint correspondance) Link
Roukos V# and Misteli T# (2014) The biogenesis of chromosome translocations. Nat Cell Biol, 16:293–300 (#indicates joint correspondance) Link
Roukos V and Misteli T (2014) Deep Imaging: The next frontier in microscopy. Histochem Cell Biol, 142:125–131 Link
2013
Roukos V, Burman B and Misteli T (2013) The cellular etiology of chromosome translocations. Curr Opin Cell Biol, 25:357–364 Link
Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D and Misteli T (2013) Spatial dynamics of chromosome translocations in living cells. Science, 341:660–664 Link
Symeonidou IE, Kotsantis P, Roukos V, Rapsomaniki MA, Grecco HE, Bastiaens P, Taraviras S and Lygerou Z (2013) Multi-step loading of human minichromosome maintenance proteins in live human cells. J Biol Chem, 288:35852–35867 Link
2012
Haj FG, Sabet O, Kinkhabwala A, Wimmer-Kleikamp S, Roukos V, Han HM, Grabenbauer M, Bierbaum M, Antony C, Neel BG and Bastiaens PI (2012) Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B. PLOS One, 7:e36633 Link
Stathopoulou A, Roukos V, Petropoulou C, Kotsantis P, Karantzelis N, Nishitani H, Lygerou Z and Taraviras S (2012) Cdt1 is differentially targeted for degradation by anticancer chemotherapeutic drugs. PLOS One, 7:e34621 Link
Vidi P, Chandramouly G, Gray M, Wang L, Liu E, Kim JJ, Roukos V, Bissell MJ, Moghe P V and Lelievre SA (2012) Interconnected contribution of tissue morphogenesis and the nuclear protein NuMA to the DNA damage response. J Cell Sci, 125:350–361 Link
2011
Roukos V, Kinkhabwala A, Colombelli J, Kotsantis P, Taraviras S, Nishitani H, Stelzer E, Bastiaens P and Lygerou Z (2011) Dynamic recruitment of licensing factor Cdt1 to sites of DNA damage. J Cell Sci, 124:422–434 Link
2010
Roukos V, Misteli T and Schmidt CK (2010) Descriptive no more: The dawn of high-throughput microscopy. Trends Cell Biol, 20:503–506 Link
2007
Roukos V, Iliou MS, Nishitani H, Gentzel M, Wilm M, Taraviras S and Lygerou Z (2007) Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit Brahma. J Biol Chem, 282:9346–9357 Link
2006
Nishitani H, Sugimoto N, Roukos V, Nakanishi Y, Saijo M, Obuse C, Tsurimoto T, Nakayama KI, Nakayama K, Fujita M, Lygerou Z and Nishimoto T (2006) Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis. EMBO J, 25:1126–1136 Link